Journal: bioRxiv

Article Title: IR-B deficiency and fatty acid dysregulation accelerate prostate cancer progression via PI3K/AKT signaling

doi: 10.64898/2026.01.30.702723

Figure Lengend Snippet: (A) Relative mRNA expression of lipid synthesis-related genes in DLP lobes of LL H and pLL +H mice by qPCR. (B) GPR120 expression in prostate adenocarcinoma (PRAD) stratified by nodal metastasis status, from TCGA data. (C) GPR120 expression based on Gleason score in PRAD patients, TCGA data. (D) Immunofluorescence staining of GPR120 in DLP lobes of Hi-Myc and wild-type mice. Scale bar: 50μm. (E) Western blot of GPR120, GLUT1, GLUT12, p GSK3β-S9, GSK3 β, GSK3α/β, Bcl-2, and BAX in DLP lobes of LL, pLL + , LL H and pLL +H mice. (F) Immunofluorescence of GLUT1 in DLP lobes. Scale bar: 50μm.

Article Snippet: The following primary antibodies were used: IRS, phospho-IRS1(Tyr896), GSK3β and phospho-GSK3β(Ser9), GPR120, GLUT1, Bcl-2, BAX (Abbkine, USA); IR and phospho-IR, AR, AKT1 and phospho-AKT, PI3K p85 alpha, GAPDH (Proteintech, Wuhan, China); Ki-67 (Cell Signaling Technology, USA); GLUT12 (Boster Bioengineering Co., Ltd., Wuhan); GSK3α/β and phospho-GSK3β (Y216) and phosphor-GSK3α (Y279) (Abcam,USA).

Techniques: Expressing, Immunofluorescence, Staining, Western Blot